QPCR standard curve-why there is a large gap between 10^6 and 10^5 Cts.?

I am using qPCR to amplify Shigella ipaH gene. This assay worked well in out lab previously, but now I cannot get good standard curve. My std. curve range is 10^6 to 10 gene copies, and 10^6 std....

09 September 2014 9,740 2 View