Ranjit Gulvady

3 Questions 5 Answers 0 Followers

Questions related from Ranjit Gulvady

Why is the UV absorbance spectra of dsDNA melting from my experiments not as per expectations?

Please refer to the attached Figure, which has three parts, A,B and C. I first annealed complementary ssDNA in a PCR machine. Then, I heated the annealed dsDNA from 25 deg to 75 deg until all the...

04 April 2019 2,025 3 View

Probability of hairpin vs self-dimer formation ?

I have a linear ssDNA oligo that is designed such that the ends are complementary, and conducive to hairpin formation (see attached figure). My question is, which has a higher likelihood of...

04 April 2019 2,246 5 View

Secondary structure prediction for multiple and simultaneous oligo annealing?

Hello, I am trying to do a DNA annealing as shown in the figure. As can be seen, this is a three part annealing that involves three different oligos. 1)While there are softwares to check...

04 April 2019 4,320 2 View