Ranjit Gulvady

3 Questions 6 Answers 0 Followers

Questions related from Ranjit Gulvady

Is there an upper limit to the length of a DNA loop for a hairpin to form?

I am trying to do some experiments, where my hairpin stem is 18 bp in length, but my loop itself is 125 nt long (something like the attached figure). I was wondering if it's possible at all for a...

14 May 2019 6,924 0 View

Why is the UV absorbance spectra of dsDNA melting from my experiments not as per expectations?

Please refer to the attached Figure, which has three parts, A,B and C. I first annealed complementary ssDNA in a PCR machine. Then, I heated the annealed dsDNA from 25 deg to 75 deg until all the...

18 April 2019 9,609 3 View

Digestion of small fragment of ssDNA from a larger dsDNA fragment?

I have a dsDNA fragment that is ~ 65 bp in length (see attached figure). I wish to digest 2 nucleotides (nt) of ssDNA right in the center of this dsDNA fragment (indicated by brown arrows in the...

10 April 2019 1,109 7 View