Ranjit Gulvady

2 Questions 6 Answers 0 Followers

Questions related from Ranjit Gulvady

Why is the UV absorbance spectra of dsDNA melting from my experiments not as per expectations?

Please refer to the attached Figure, which has three parts, A,B and C. I first annealed complementary ssDNA in a PCR machine. Then, I heated the annealed dsDNA from 25 deg to 75 deg until all the...

18 April 2019 9,521 3 View

Digestion of small fragment of ssDNA from a larger dsDNA fragment?

I have a dsDNA fragment that is ~ 65 bp in length (see attached figure). I wish to digest 2 nucleotides (nt) of ssDNA right in the center of this dsDNA fragment (indicated by brown arrows in the...

10 April 2019 1,073 7 View