Ranjit Kumar Das

3 Questions 4 Answers 0 Followers

Questions related from Ranjit Kumar Das

Do I need to use Fluoromount-G™ Clear Mounting Media as mounting the coverslip for Immunohistochemistry ?

We would like to see the protein expression for (IFIT3 gene which is nuclear protein) using Immunohistochemistry. Do I need to use Dapi-Fluoromount during the mounting steps. Here I have attached...

19 March 2023 5,152 3 View

Western blot BCA protein estimation and denature ?

Can someone please help me to calculate protein concentration using BCA standard curve, how to dilute samples and how much 4x Laemmli should I add to denature the protein? I have been struggling...

14 March 2023 7,828 4 View

No band shows in western blot ?

I have been doing western blot for STAT1 gene. I have tried primary antibody from different companies Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 ( Cell Signaling Technology),Phospho-STAT1...

14 January 2023 4,641 3 View