Rania Faidi

4 Questions 16 Answers 0 Followers

Questions related from Rania Faidi

Has anyone used TMEM119 alongwiith Cd11b/Cd68 to stain inflammatory microglia in mouse frozen sections?

Hi everyone, I'm interested in the anti-TMEM119 antibody from Abcam (ab209064) to immunohistochemically label microglia in frozen mouse sections. Has anyone used this antibody from abcam and...

04 April 2019 5,116 11 View

What is the recommended thickness of brain slice for microglia immunohistochemical staining?

Hi everyone I am new to IHC. I have been trying to understand if the thickness of the tissue really dictates how much morphology one can detect from immunohistochemical staining of microglia (if...

02 February 2019 1,710 12 View

Do I need to buy vacuum filters when culturing primary mixed glia and primary microglia?

Hi everyone I am interested in learning about your experience with vacuum filters for cell culture. I have never come across needing them when working with HEK, RK or SHSY5Y cells but I recently...

11 November 2018 1,160 4 View

Can anyone suggest a technique to temporarily store brain tissue if my end goal is to grow primary microglia cultures?

Hi, I am interested in examining the effects of an endotoxin on microglial cells. I will be harvesting brains from mice at p2, treating them with this factor and then performing some molecular...

09 September 2018 2,282 3 View