Rania Magadmi

5 Questions 5 Answers 0 Followers

Questions related from Rania Magadmi

Does anyone have experience in block mouse cultured dorsal root ganglion?

I'm running experiments and I need to silence the mouse DRG in culture. I'm thinking of either Lidocaine or Toxin cocktail (TTX with conotoxins). Does any one have experience in this and willing...

30 November 2015 5,891 4 View

How can I knockdown a gene in BMMC cells?

Hello all,I would like to knockdown some gene in mouse bone marrow derived mast cell to produce a stable expressing. Could you suggest me an appropriate method to do? After knockdown, I also want...

19 June 2015 6,693 1 View

What is a good functional assay for sensory neurons?

I would like to measure DRG response using Elisa. Im thinking to measure GABA, Glutamate and CGRP. what else do you think is important to measure too? and any one try to measure those mediators...

01 April 2015 238 3 View

How do you seed BMMC on glass coverslip for ICC or live cell imaging?

I'm working on BMMC and I have problems with seeding BMMC on glass coverslip to use it later for ICC or live cell imaging. I tried Matrigel BD 356235 con.1:5. I coated the coverslip with...

26 September 2013 10,001 1 View

Gene microarray for co-culture model?

I'm going to use microarray technology (Affymetrics) to study gene expression of 2 different cell types in coculture model . Does anyone know of a way to separate two cells or identify them to...

16 May 2013 9,296 10 View