Rania Faidi

2 Questions 7 Answers 0 Followers

Questions related from Rania Faidi

How do you avoid knife tracks from a sliding microtome?

Hi everyone, I am using a sliding microtome to slice some brains I have been collecting for the purpose of immunofluorescence (the brains have been PFA/PBS perfused, then post-fixed overnight...

04 February 2020 7,205 4 View

Can anyone suggest a technique to temporarily store brain tissue if my end goal is to grow primary microglia cultures?

Hi, I am interested in examining the effects of an endotoxin on microglial cells. I will be harvesting brains from mice at p2, treating them with this factor and then performing some molecular...

28 September 2018 1,885 3 View