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Questions related from Randy Chance
I use the same primer set and same conditions. The first PCR product from cDNA gave about 800 bp. Reamplification of the template gave 700 bp. A second amplification gave 600 bp. Interestingly, I...
02 February 2020 3,373 8 View
An insert length of 811bp (17ng/ul) and digested vector size of 5.6kb (35ng/ul). --100ng vector amount--. Am I right in using the following respective volumes of insert and vector? 2.5ul and...
05 May 2019 1,698 3 View
