Randula Fonseka

3 Questions 1 Answers 0 Followers

Questions related from Randula Fonseka

Uncut plasmid runs slower than cut plasmid in agarose gel?

I compared my uncut plasmid to the same plasmid cut with a single restriction enzyme that is a single site cutter and the uncut plasmid ran in the gel slower. The size of my cut plasmid is around...

14 September 2019 5,836 2 View

What does a decrease in LC3-II mean in terms of autophagy?

I'm trying to figure out if my protein of interest, Protein X, which is a viral protein, causes autophagy. I am probing for human LC3B using a polyclonal rabbit antibody in a human endothelial...

26 March 2019 6,028 2 View

Why protein in Western blot is higher than expected molecular weight?

I am probing for human LC3B which has two bands with molecular weights of 16 and 14 kDa. However when I image the membrane I see the 16 and 14 size bands but also two more bands around 28 kDa and...

25 March 2019 5,418 4 View