Rana Mumtaz Raoof

7 Questions 19 Answers 0 Followers

Questions related from Rana Mumtaz Raoof

What is the best statistical test that can deal with large data sets?

Dear All: I am wondering about the best statistical test that can deal with large data sets. I am profiling plasma microRNAs using a high throughput method and comparing the expression of those...

06 June 2016 7,628 5 View

Can anyone advise about what data to plot in the ROC curve to obtain the sensitivity and specificity of a microRNA as a disease biomarker?

Dear All: can anyone advise about what data to plot in the ROC curve to obtain the sensitivity and specificity of a microRNA as a disease biomarker? we are validating our target microRNAs...

01 January 2016 9,908 12 View

Can anyone help analysing microRNA microarray data?

I am doing an experiment to check plasma microRNA expression profile in two time points in healthy individuals by using AB openarray protocol. In some samples the raw (time0) ct values are...

05 May 2014 7,036 4 View

What is the minimum amount of sample to start an RNA sequencing experiment (for both human and mice blood samples)?

11 November 2013 3,205 3 View

Normalization of microRNA profiling study in plasma samples?

I am wondering what is the best method for normalization of microRNA in RT-qPCR experiments. My samples are plasma samples so can I use a reference gene for normalization? And what is the most...

11 November 2013 5,750 3 View

Using Nanodrop 2000 to detect blood sample hemolysis?

Can any one advice me on the way to perform spectrophotometric analysis on blood samples to check for hemolysis using Nanodrp 2000? We need to measure absorbance at 414 but because I am not...

11 November 2013 6,369 8 View

Can someone suggest a sample transport in liquid nitrogen?

My work involves transportation of biological samples from the hospital to the lab. I was wondering about the best liquid nitrogen container to do that, my samples are small so a 1-2 L containers...

10 October 2013 4,330 16 View