Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

16 September 2020 5,466 3 View