Ran An

2 Questions 6 Answers 0 Followers

Questions related from Ran An

Tissue PCR didn't work while cell PCR worked well?

I did PCR with my cell samples, and it worked very well. Please see 28S of my cell samples in Figure 1. But my tissue samples did not work well, please see 28S of my tissue samples in Figure 2....

03 March 2018 6,259 5 View

Getting precipitate or milky solution (without any precipitate after centrifuge) after acidification of digested cell lysates?

I am preparing samples for phosphoproteomics. After reduction, alkylating, and digestion, I acidified my cell lysates with 20% TFA before purifying the digest with Sep-pak. When I digested ~1.5mg...

01 January 2018 9,313 4 View