Ramya Ganiga Prabhakar

3 Questions 4 Answers 0 Followers

Questions related from Ramya Ganiga Prabhakar

Does Lysozyme cause the lysis of cell wall as well as inner membrane in gram positive bacteria?

Hi, I am trying to lyse gram positive E. faecalis using lysozyme (final concentration: 100ug/ml). I suspend the bacterial pellet with lysozyme in buffer for 30min at 37C. Then run the supernatant...

05 May 2016 9,049 14 View

Does J774A.1 form clumps in cell culture flask?

I see the newly thawed J774A.1 cells form clumps of about 10-20 cells. On sub-culturing the size of the clump reduced to about 5-10. Is that normal? I am using DMEM media supplemented with 10%FBS...

01 January 2016 6,540 6 View

What is the difference between a semi-quantitative PCR and qPCR?

Is there a difference in the mechanism or the method?

11 November 2015 2,090 2 View