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details: vector and insert are 2.8 Kb, both spin coloumn purified and REN checked. REN sites were introduced into insert (with 5bp extra sequences at 5`end). O/N REN digestion followed by CIP...
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The same set of PCR conditions including the cDNA tube once gave nice clear bands, but two days later there were absolutely no bands at all (i have repeated this twice). This pattern has occurred...
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