Ramya Ganiga Prabhakar

2 Questions 2 Answers 0 Followers

Questions related from Ramya Ganiga Prabhakar

Does Lysozyme cause the lysis of cell wall as well as inner membrane in gram positive bacteria?

Hi, I am trying to lyse gram positive E. faecalis using lysozyme (final concentration: 100ug/ml). I suspend the bacterial pellet with lysozyme in buffer for 30min at 37C. Then run the supernatant...

09 May 2016 482 14 View

Does J774A.1 form clumps in cell culture flask?

I see the newly thawed J774A.1 cells form clumps of about 10-20 cells. On sub-culturing the size of the clump reduced to about 5-10. Is that normal? I am using DMEM media supplemented with 10%FBS...

11 January 2016 2,301 5 View