Ramsey Najm

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Questions related from Ramsey Najm

Any suggestions on how to reduce polymerization of Matrigel after harvesting iPSCs for neuronal differentiation?

I am culturing iPSCs on matrigel coated plates using mTeSR media. When I manually lift off iPSC colonies for neurosphere formation with the intent of further differentiation into neurons I get a...

25 July 2015 8,206 2 View

Does anyone have a general protocol on how to prepare cell culture media for an ELISA assay, specifically for secreted proteins in vitro?

I need to do an ELISA assay for secreted Aß oligomers in the near future and was curious if anyone had a general protocol on how to prepare and store the cell culture media. I've seen some online...

18 March 2015 8,596 3 View

Is a certain level of differentiation normal upon in-vitro lentiviral transduction of non-targeting shRNA in human ES cells?

I am infecting human ES cells with a lentiviral construct containing shRNA and am using a non-targeting vecor as control. However, in my non-targeting control I see a high level of differentiation...

29 September 2014 5,118 3 View

Lentiviral Transduction of H9 human ES cells

I am transducing H9 human ES cells with a lentiviral shRNA vector that contains a CMV promoter controlling shRNA and a tGFP marker. For some reason I see no GFP expression in my ES cells, but...

20 August 2014 5,936 1 View