Ramachandra Nagaraju

4 Questions 4 Answers 0 Followers

Questions related from Ramachandra Nagaraju

Can i use yeast episomal plasmids to integrate into yeast genome for expression?

I am finding difficult to clone an ion-channel gene in yeast episomal expression vector. All attempts to clone in parts and then subclone to get full length clone also was not successful. So i...

05 May 2016 9,279 7 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

01 January 2016 7,272 2 View

Please suggest for native page western blotting??

I want to perform a western blot of a native page. I tried with normal tris transfer buffer with methanol and it did not work. Since it is a native page, i wonder there is no SDS to charge the...

10 October 2015 1,252 2 View

Is it possible to express two proteins under one strong promoter (GAL or LacZ) in a plasmid?

Hi, I would like to know whether is it possible to overexpress two different proteins under a single strong promoter in plasmid. In such a case which expression system is ideal and in this case...

01 January 2015 6,604 6 View