How do I calculate IC50 of Cu superoxide dismutase using NBT assay?

I want to Calculate IC50 value. O2 . - conc, NBT conc at PH 7.3, Cu-SOD conc all are known. I know that NBT on reaction with superoxide(SO2.- )  form MF+ with Abs  at 520 nm.But what is the...

17 July 2015 9,481 4 View