Ralitsa Radostinova Madsen

3 Questions 12 Answers 0 Followers

Questions related from Ralitsa Radostinova Madsen

What posttranslational modification may result in a large basic shift in a protein's pI only after 10 min stimulation with ligand and no mass change?

I am running 2D westerns as part of a study on receptor signalling and posttranslational modifications. In my particular case, a subspecies of our receptor of interest is generated upon ligand...

06 June 2015 9,023 7 View

Does anyone have any experience with using TAT-Cre that they would like to share?

I am about to start some new experiments that rely on TAT-Cre (from Millipore) to delete a gene of interest in cell culture in order to study the impact on cell differentiation and gene...

05 October 2014 6,552 5 View

Microarray normalisation strategies - can anyone provide an explanation?

I have used Agilent's miRNA microarrays to profile miRNA expression in young vs old adipose tissue. The analyses were performed externally, and I am trying to understand the underlying...

03 July 2014 1,170 5 View