Ralf Kölling

2 Questions 4 Answers 0 Followers

Questions related from Ralf Kölling

Is there any evidence that the Phos-Tag binds to other groups than phosphate?

My protein shows several shifted bands on Phos-tag gels. Only one of these bands can be removed by treatment with alkaline phosphatase, the other bands are resistant to phoshatase treatment. Also,...

22 December 2024 4,620 2 View

What EXACTLY happens in the stacking gel during SDS PAGE?

I have the problem, like several other people before, that large proteins get lost during electrophoresis in the SDS PAGE gel. All protein bands below 80 kDa are perfectly separated as usual, the...

18 October 2021 6,053 8 View