Rakhe Tepin

7 Questions 17 Answers 0 Followers

Questions related from Rakhe Tepin

Can i restart the PCR where i have put wrong temperature?

In 35 cycle at 94degree C, 54degree C and 72 degree C by mistake in place of 54 degree I did 5.4 degree C and it ran for almost 25 cycle. Can i restart the PCR and what will happen if i did? Thank...

17 June 2024 6,131 3 View

What are unpublished DNA sequences in NCBI?

I have seen a lots of unpublished DNA sequences in NCBI. Is it authentic to used them in a research paper? What are those sequences?

31 July 2023 5,513 7 View

What would happen if I run pcr once again (two times)?

After running pcr reaction (for 35 cycle) the dna concentration i got was low. Is it possible to use same tube to run it again (for 35 cycle or any)? what would happen if i do that?

17 June 2023 3,301 6 View

Problem in DNA sequencing or PCR?

I have sent amplified DNA (ITS region) using primer pair ITS1 and ITS4 for sequencing in a company that provide sequencing service but they are able to successfully sequence either ITS1 or ITS4 in...

16 June 2023 6,149 6 View

What to do with this kind of sequences?

I want to do a Phylogenetic analysis to classify organisms using following sequences. I have download some sequences (ITS region) from NCBI database and aligned them but because of few sequences...

25 April 2023 7,666 5 View

What is this structure?

Found this structure in the hypha of a species of Hymenochaete. It was quite frequent in the context. Is it common for such structure to appear? if yes, than can someone please provide me the...

13 February 2023 5,515 2 View

Which region is called cortex in Hymenochaete species?

Can anyone provide me some pictures or diagram to show the region called "cortex" in Hymenochaete species and/or some description about the region and how to identify if a species of Hymenochaete...

18 November 2022 3,251 2 View