Rakhe Tepin

5 Questions 11 Answers 0 Followers

Questions related from Rakhe Tepin

What are unpublished DNA sequences in NCBI?

I have seen a lots of unpublished DNA sequences in NCBI. Is it authentic to used them in a research paper? What are those sequences?

31 July 2023 5,505 7 View

What would happen if I run pcr once again (two times)?

After running pcr reaction (for 35 cycle) the dna concentration i got was low. Is it possible to use same tube to run it again (for 35 cycle or any)? what would happen if i do that?

17 June 2023 3,283 6 View

Problem in DNA sequencing or PCR?

I have sent amplified DNA (ITS region) using primer pair ITS1 and ITS4 for sequencing in a company that provide sequencing service but they are able to successfully sequence either ITS1 or ITS4 in...

16 June 2023 6,143 6 View

What to do with this kind of sequences?

I want to do a Phylogenetic analysis to classify organisms using following sequences. I have download some sequences (ITS region) from NCBI database and aligned them but because of few sequences...

25 April 2023 7,657 5 View

Which region is called cortex in Hymenochaete species?

Can anyone provide me some pictures or diagram to show the region called "cortex" in Hymenochaete species and/or some description about the region and how to identify if a species of Hymenochaete...

18 November 2022 3,242 2 View