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Questions related from Rakesh Maharjan
I am trying to collect the cryoEM data of a protein. The protein is ~25 kDa but oligomerizes with 10 or 12 molecules forming a ring of homodecamer or homododecamer. In the negative staining with...
26 November 2020 5,684 5 View
I am doing crystallization of a protein. I have tried many screening kits but couldn't get crystals. The theoretical pI of protein is 5.9 and the pH of buffer I am using is 8.0 (20mM tris-hcl,...
06 June 2018 6,688 17 View
Recently, I did crystallization screening of my protein after purification and few conditions produced crystals, some of which were large and confirmed to be protein crystals. However, I am not...
30 November 2017 2,337 9 View
I cloned my gene of interest in pET-22b vector. I could transform DH5α E. coli strain. But I am not being able to transform expression strain of E. coli. My transformation protocol is; thaw the...
10 March 2017 1,898 5 View
