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Questions related from Rajendra Pangeni
I transfected GipZ lentiviral shRNA with packaging plasmids psPAX2 and VSVG into 293T cells. I also transfected LentiCRISPRv2GFP (lentiviral based GFP containing CRISPR from Adgene). The...
01 January 2018 5,661 2 View
hi guys, I tried to transfect lentiviral based shRNAs into 293T cells and subsquently infect into stem cells. My transfection and infection looks 70% and 90% positive respectively based on GFP...
03 March 2016 3,945 3 View
I would like to look at the expression of a single miRNA. At this point, I would be inclined to look at miRNA expression by general RT (similar to RT for any other mRNA for a gene) without going...
09 September 2014 7,003 4 View