Rajdeep Chowdhury

8 Questions 10 Answers 0 Followers

Questions related from Rajdeep Chowdhury

Feedback about Thorlabs adaptive optics module?

Hi all, I am planning to purchase an adaptive optics from Thorlabs...Main purpose is to get astigmatism...There are various options in terms of wavefront sensor (CCD or CMOS), number of actuators...

06 June 2018 5,855 0 View

Some fraction of protein leaving behind the Ni-NTA resin even after eluting with 250 mM imidazole?

I purified a C-terminally His-tagged nanobody using Ni-NTA. After labeling the nanobody with a alexa dye, when I was trying to get rid of the free dye using Ni-NTA, I found ~30% of labeled...

01 January 2018 8,184 6 View

What is the most efficient and faster way to generate a stable cell line starting from a plasmid?

I use lipofectamine 2000 to transiently transfect my plasmid into the HeLa cells and then use selection antibiotic to make stable cell. This method does not give very good transfection efficiency...

07 July 2017 444 1 View

How to fit a fluctuation data with change point algorithm?

I have a data of fluorescence intensity (I) which shows a nice wavy pattern with time (t). I want to fit this I vs. t data to change point algorithm. If anyone has any idea about the matlab code...

05 May 2017 9,437 4 View

Using Matlab how to sort a matrix based on the value of elements of 1st column?

I have a 1000 by 2 matrix. I want that whenever there is an element >100 in the 1st column that row will be deleted. In a more simpler way, 1st column is the x-values and 2nd column is...

04 April 2017 5,973 4 View

Is inverse PCR the only option to mutate one central base of a plasmid?

I have a plasmid which differs by one base from wild type. This base will change the protein by one amino acid, hence I need to mutate that base which is residing in the central part of the...

03 March 2017 7,132 3 View

Protein expression?

I working on the expression of a protein in BL21 cell using IPTG as inducer. Usually after adding the inducer, overnight culture is suggested followed by centrifugation to pallet down the cells. I...

01 January 2017 8,114 8 View

Ideal colony size during plasmid transormation in BL21 (DE3) cells?

When I am transforming a plasmid in BL21 (DE3) cells, plate is found to contain colonies of different size. Roughly they may be classified as small, medium and large. Are they all equivalent for...

01 January 2017 9,211 3 View