Rajarshi Roy

4 Questions 3 Answers 0 Followers

Questions related from Rajarshi Roy

How to stop protein loss after dialysis?

Hello , After I have affinity (6x his pet24) purified protein from inclusion body using 50mM tris ph8 , 300mM Nacl , 250mM imidazole and 8M urea I have subjected it to dialysis in 50mM tris pH 7.6...

02 August 2023 6,532 2 View

How to stop protein from precipitation during concentration?

Hello, While concentrating my GST-tagged protein in a Merck 10kDa cutoff Centricon , the protein appears to be aggregating/precipitating. As is understandable when there is turbidity/whitish...

23 March 2023 8,460 1 View

Is it possible to raise antibodies using GST-tagged chimeric proteins?

Is it possible to raise antibodies using GST-tagged chimeric proteins? And also using only GST protein as a control?

12 December 2022 4,073 3 View

How to separate protein of interest from GST-tag with Thrombin?

Hello all, I am facing an annoying problem wherein I am attempting to remove my GST tag from my (glutathione eluted) chimeric protein using a Thrombin Cleavage kit (Biotinylated Thrombin), but...

06 December 2022 3,840 3 View