Raja Sekaran

3 Questions 5 Answers 0 Followers

Questions related from Raja Sekaran

How could I prepare oleic acid standard?

Can anyone suggest how do oleic acid standard for lipase assay, olive as substrate? I bought oleic acid from merck. Description of oleic acid ,M=282.47 g/mol, (1l=0.89kg) specifiction: Assay...

12 May 2015 3,571 6 View

Can anyone explain the role of pyridine in cupric acetate pyridine for the lipase assay?

Is that Pyridine play only important role in pH adjustment or any other else in preparation of Cupric acetate pyridine for lipase assay?

11 May 2015 9,014 5 View

Can palm oil be used as a substrate in lipase enzyme assay and palmitic acid as a standard?

In general lipase protocol- Lipase activity assayDetermination of liberated free fatty acid was measured by colorimetry method (Kwon and Rhee, 1986) using olive oil as substrate. The reaction...

09 April 2015 8,825 3 View