Raja Ms

3 Questions 2 Answers 0 Followers

Questions related from Raja Ms

What are the eithical considertion for working with SiHa and SkOV3 cell lines for assessing the drug response in cervical cancer or ovarian cancer?

their impact to care diagnosis and delays IRAC ethics GDPR

22 April 2022 5,099 0 View

Amplifiction of full length gene + Primers with Re Sites of enzymes + Tm ?

I want to amplify 3.4 kb gene fragment with primers having the restriction sites of enymes which i will use further. before adding the restriction sites Tm of my forward primers is 54.4 and after...

09 March 2021 1,276 1 View

Should i use Normal taq polymerase for the synthesis of 3.4kb gene fragment of phusion taq polymerase (thermo scientific)?

i am trying to amplify the 3.4 kb gene with primers having 5" Re Sites added. but not getting result neither with taq pol nor phusion enyme. even dimers are not present in the gel? i am very...

09 March 2021 4,178 0 View