We tried to do ddPCR (in duplex) with two FAM probes and one HEX probe. In the 2D plot there appear two WT clusters. Why is this?

we created a duplex ddPCR containing two FAM probes of different mutations and one HEX probe for one of these two mutations. when analyzing the results, two mutant clusters were shown in the 2D...

16 July 2018 1,414 5 View