Rais Ahmed

6 Questions 6 Answers 0 Followers

Questions related from Rais Ahmed

Is there any research article published that indicate bacterial DNA degradation/survival in soil?

dear all scientists, i am in search of an article that contain information about Bacterial DNA degradation/ survival in soil with effect of different temp, pH, humidity etc...please inform if...

04 August 2016 2,460 2 View

Vaccinated animal with RB51 vaccine, show PCR positive?

If we vaccinate the healthy animals with Brucella live vaccine RB51, then what is the possibility that PCR result will be positive or negative?

09 February 2016 3,006 2 View

What are the basic steps to optimize real time PCR?

how to optimize real time PCR??

17 December 2015 3,641 4 View

Any advice on the problem in optimization of real time PCR?

i am facing problem in real time PCR optimization. i am using Bio Rad system CFX96. I found Tm of primers 54C in gradient PCR. then i run real time PCR but no peaks are seen but i found clear...

26 November 2015 2,027 3 View

Are you familiar with template DNA quantity in qPCR multiplex?

While using multiplex real time PCR, what should be the exact quantity of target/template DNA? Either it should be the same as in real time PCR (not multiplexed) or it should be high in case of...

15 May 2015 2,753 3 View

Are you familiar with probe designing in real time multiplex PCR?

I would like to know that what should be kept in mind while designing probes for real time multiplex PCR and machine used is Bio-rad CFX96 real time PCR system?

15 May 2015 6,576 2 View