How do you standardise long range pcr which is a pentameric AT rich repeat region and expansion along with an insertion?

Hello all, I am trying to do a PCR for a repeat rich region (AT rich) which gets expanded. I have tried using normal Thermo Taq Pol, LA taq from Takara. However I am not able to amplify the longer...

10 October 2018 4,800 9 View