Rahul Jain

3 Questions 6 Answers 0 Followers

Questions related from Rahul Jain

Any tricks to increase conjugation frequency in Gammaproteobacteria using Ecoli S17-1 donor harboring Tn5 based fluorescent plasmids?

I am trying to label some of the bacterial endophytes (Pseudomonas, Serratia, Pantoea, Acinetobacter, Sphingomonas and Arthrobacter) for performing in-planta studies. I have chromatic plasmids...

26 April 2021 704 1 View

Can ABTS assay used for laccase enzyme be positive due to some other low molecular weight compounds/protein?

I am working on laccase enzyme from a bacteria which is very active in low temperature. While running the crude enzyme on native PAGE for zymogram analysis using ABTS, active bands are visible...

17 May 2016 6,655 14 View

How can I break aggregated protein (enzyme) for its purification?

I am working on cold active lipases and having trouble in purifying the enzyme due to very high degree of aggregation. I have tried breaking the aggregates using several detergents such as CHAPS,...

01 January 1970 10,056 10 View