Rahul Jain

5 Questions 7 Answers 0 Followers

Questions related from Rahul Jain

Any tricks to increase conjugation frequency in Gammaproteobacteria using Ecoli S17-1 donor harboring Tn5 based fluorescent plasmids?

I am trying to label some of the bacterial endophytes (Pseudomonas, Serratia, Pantoea, Acinetobacter, Sphingomonas and Arthrobacter) for performing in-planta studies. I have chromatic plasmids...

26 April 2021 755 1 View

Any one using mPNA and pPNA clamps for metagenomics studies?

I am interested in doing microbial diversity analysis associated with a plant. I was looking for PNA clamps suppliers who can provide me mitochondrial PNA and plastid PNAs. Let me know if anyone...

10 December 2019 4,489 1 View

Can ABTS assay used for laccase enzyme be positive due to some other low molecular weight compounds/protein?

I am working on laccase enzyme from a bacteria which is very active in low temperature. While running the crude enzyme on native PAGE for zymogram analysis using ABTS, active bands are visible...

17 May 2016 6,714 14 View

Is there a method to disaggregate lipase from pseudomonas?

I m purifying it using IEx (DEAE) and GF chrm but not getting success cz of aggregation. In ion exchange the enzyme is getting eluted at low salt concentration of 0.2M while in GF chrom (Sephadex...

25 January 2016 6,113 4 View

How can I break aggregated protein (enzyme) for its purification?

I am working on cold active lipases and having trouble in purifying the enzyme due to very high degree of aggregation. I have tried breaking the aggregates using several detergents such as CHAPS,...

01 January 1970 10,108 10 View