How many pre-B cells are needed to obtain 1-2 mg of protein?

I am performing a phosphor-proteomics project and need 10-20 mg/ml protein in 100-200 ul buffer.  I am using an 8M Urea buffer and sonication (15-20 2sec bursts) for lysis. I performed a test run...

09 July 2015 4,838 1 View