Rahul Bhansali

2 Questions 2 Answers 0 Followers

Questions related from Rahul Bhansali

What is a good fractionation protocol for a kinase assay and subsequent mass spec analysis?

I am deciding whether or not to fractionate my cell lysate to perform a kinase assay for mass spec analysis of direct targets (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3131159/). The above...

18 August 2015 824 4 View

How many pre-B cells are needed to obtain 1-2 mg of protein?

I am performing a phosphor-proteomics project and need 10-20 mg/ml protein in 100-200 ul buffer. I am using an 8M Urea buffer and sonication (15-20 2sec bursts) for lysis. I performed a test run...

09 July 2015 4,851 1 View