Ragini Kumari

2 Questions 2 Answers 0 Followers

Questions related from Ragini Kumari

My malachite control reaction mix (without enzyme) shows absroabnce ~ 1.5. What can be the reacon?

I am trying to access the GTPase activity of my protein using malachite green kit by sigma. According to the protocol mentioned, after adding malachite it should be incubated for 30min. In 30...

23 January 2025 5,982 1 View

I am trying to assess the GTPase activity of my enzyme. I am getting high absorbance from GTP solution. Is there a way to otpmize that?

The GTP that I am using is trisodium salt obtained from sigma. To prepare the GTP solution, I am dissolving it in 50mM Tris, pH adjusted to 7.5. The solution is sterile filtered, aliquotted and...

04 October 2024 2,574 2 View