Raghumoy Ghosh

2 Questions 12 Answers 0 Followers

Questions related from Raghumoy Ghosh

Is trypan blue toxic to zebrafish cells in general ?

So I have been trying to standardise a zebrafish single cell suspension for downstream sequencing. I am consistently getting decent viability if I measure it via syto 9 and propidium iodide...

29 March 2023 5,027 2 View

Techniques for targeted knock in of small nucleotide in zebrafish (<20bp) in vivo?

I want to introduce the sequence for a tetracysteine tag in a protein of interest, in vivo. The nucleotide sequence is just 18bp long. I know I can use Crispr to knock-in genes but most papers are...

01 March 2021 8,275 2 View