I am having trouble with getting a good PCR result, my expected PCR product is 2355 bp , what should I do?

I am using the promega green master mix  and the program I used: 1- 94C for 5 min 2- 45 cycle of 94C: 1:30min, anealing at 60.5C :30s , extetion at 72C : 2:30min 3- 72C for 10 min I tried to...

11 November 2014 2,147 21 View