Raghavendra Basavaraja

4 Questions 20 Answers 0 Followers

Questions related from Raghavendra Basavaraja

Hi, Does anyone have a good protocol for miRNA pulldown assay? Are there commercial kits available?

We are looking for microRNA pull down assy for human cell lines.

09 September 2019 8,811 4 View

What are the positive and negatives about using mRNA transfection over plasmid or other method?

We are planning to over express a particular protein in endothelial cells. Would like to hear comments from an experienced people with mRNA transfection kits and conditions .

11 November 2017 607 3 View

Looking for methods to culture CL tissues except micro dialysis?

We would like to test some drugs effect on CL. Can you pl suggest me a suitable methods to isolate and culturing of CL?

10 October 2014 9,228 1 View

What is the Ideal Slope and R^2 value for a gene primers in qPCR Standard curve analysis?

I got a slope of -3.9473 and r^2 value of 9.4 am worrying about its efficiency because I heard -3.32 is ideal slope .

05 May 2014 3,140 4 View