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Questions related from Raghav Oberoi
I am facing some problems in dissolving statins. We want to administer statins to the mice via drinking water. The statin we are using are clinical grade from the pharmacy. First I tried to...
29 September 2016 5,155 4 View
I need to isolate the RNA from different sub-populations of human PBMCs. From the smallest population, I have cell number varying between 5000-12000 cells. The final end point of the study is to...
08 July 2015 457 5 View
I am trying to establish intra-hepatic bone marrow transplant protocol. I have to use intra-hepatic injection in new born mice pups. in order to just have hand on the injection, the new born pups...
28 November 2014 3,613 6 View
I am having problem in differentiation of THP-1 monocytes into Macrophages. THP-1 cells are growing fine. I plate them into, 6 well plate at a concentration of 1X10^6 /ml. The final PMA...
11 February 2014 6,305 5 View
Is it important to coat the flask / tissue culture plates etc with ECM like gelatin or collagen while culturing HUVEC or doing experiments with HUVEC. My cells adhere much better when I culture...
30 August 2013 5,227 6 View
I isolated LDL from human plasma by ultra centrifugation followed by dialysis overnight. Now I want to oxidize a part of the native LDL with CuSO4 but 24 hrs after oxidation at 37 degrees, I can't...
28 May 2013 3,099 25 View
Why is that Apoe KO mice develop plaques even when fed on normal chow diet as compared to ldlr KO mice which only develop plaques when fed on high fat diet?
17 February 2013 6,604 0 View
