Rafika Nanda Putri

2 Questions 3 Answers 0 Followers

Questions related from Rafika Nanda Putri

How to determine LOD and LOQ value of qRT-PCR using probit regression ?

can someone explain me how to determine LOD and LOQ value of qRT-PCR using probit regression ? i've calculated LOD and LOQ value of my qRT-PCR result using regression linear, but in some...

16 August 2021 9,392 2 View

Is this okay if i used probe for qRT-PCR that has Tm value slightly different from the probe ?

I have designed primers and probes for my qRT-PCR experiments. But the probe that I chose has a Tm value that is only slightly different from the primer, some even have the same, is this okay?

07 July 2021 6,969 4 View