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Questions related from Rafika Nanda Putri
can someone explain me how to determine LOD and LOQ value of qRT-PCR using probit regression ? i've calculated LOD and LOQ value of my qRT-PCR result using regression linear, but in some...
16 August 2021 9,451 2 View
I have designed primers and probes for my qRT-PCR experiments. But the probe that I chose has a Tm value that is only slightly different from the primer, some even have the same, is this okay?
07 July 2021 6,988 4 View
Because i want to follow the receipt mastermix from Sensifast Probe No-ROX One-step kit, in the protocol they suggest using a probe with a concentration 10 uM, but I only have probes in stock with...
05 June 2021 956 3 View