Rafal Czapiewski

3 Questions 7 Answers 0 Followers

Questions related from Rafal Czapiewski

How many ug of nuclear extract should I use for EMSA?

I am trying to optimize my EMSA conditions. What is the amount of proteins I should try first?

10 October 2013 349 0 View

Transfection of HEF and BJ cells with Lipofectamine 2000

Has anybody transfected BJ and HEF cells with Lipofectamine 2000? If yes, what was your DNA:Lipo condition? I want to start optimization with a starting range for a 24 well plate.

02 February 2013 9,068 5 View

Why does an empty vector (promoterless) show higher luciferase activity than a vector with my promoter of interest (pGL4.10)

I am trying to test regulation of the promoter of some genes by luciferase assay. I cloned this promoter into pGL4.10 and transfected HEK293T cells. As a control I used empty plasmids. Why did...

02 February 2013 5,647 7 View