Rafael Sesmero

4 Questions 6 Answers 0 Followers

Questions related from Rafael Sesmero

Which are the molecular weights of 18S and 28S rRNA in plants?

After a RNA extraction, 1% agarose gel is normally performed to verify the quality/integrity of the RNA. It seems like RNA extraction is fine as far as two bands appear (with no smear),...

05 May 2015 5,000 4 View

How can I get rid of polyphenols in olive leaves RNA extraction?

RNA extractions of olive leaves using trizol method (followed by purification with Phenol: cloroform: isoamylic alcohol 25: 24: 1 and precipitation with AcNa 3M) sometimes yield brownish-greenish...

04 April 2015 6,633 7 View

Does the q-PCR efficiency depend on the cDNA samples?

I have had some preliminary evidences showing different q-PCR efficiencies (HKG and target genes) for different cDNA samples, using the same pair of primers. I reckon that, theoretically,...

03 March 2015 8,363 6 View

Is it true that stepping on the cucumber plants may cause the fruits to go sour?

This affirmation is claimed by many farmers in my hometown. Although I've seen no scientific evidence in bibliography, it seems sound to me since many plants reacts to mechanical stress by...

07 July 2014 7,022 0 View