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Questions related from Rafael Sandoval
I want to synchronize mouse embryonic stem cells in G2/M phase in order to study transcription of a gene of interest during mitosis and throughout the cell cycle but I'm a having a hard time...
01 April 2021 8,258 2 View
Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...
19 February 2021 4,161 3 View
Hello, I'm interested in determining the size profile of nascent RNA from the pericentromere in mouse embryonic stem cells. I would like to use the Click-iT Nascent RNA Capture Kit to enrich for...
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