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Questions related from Rafael Brandão
I have been sorting NIH 3T3 based on GFP positivity for quite a long time and this has worked fine when all the GFP positive cells are sorted to the same well (of a 24 well or 12 well plate). The...
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I'm running several ELISA tests at the moment and it seems that sometimes they work properly and sometimes they don't. I coat overnight (4ºC), then the next day I block and incubate with primary...
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I'm running some ELISA experiment plates coated with an extremely expensive protein. However, at the end of the experiment there was no signal. The last step of this experiment was the incubation...
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