Rae Farnsworth

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Questions related from Rae Farnsworth

Will a luciferase promoter activity assay work if the test cells don't endogenously express the gene of interest?

I am currently working up a set of experiments to determine the signaling pathways regulating transcriptional control of our gene of interest. We already have full length and truncated promoter...

02 June 2016 9,516 1 View

Does anyone have experience using cold-active proteases to digest tissues for single-cell RNA-Seq?

I'm hoping to get a cold digest protocol working in my laboratory to perform single-cell RNA-Seq on various mouse tissues, based on e.g. Potter et al. 2019 Methods Mol. Biol, digestion of mouse...

01 January 1970 3,951 2 View