Rae Farnsworth

3 Questions 3 Answers 0 Followers

Questions related from Rae Farnsworth

Will a luciferase promoter activity assay work if the test cells don't endogenously express the gene of interest?

I am currently working up a set of experiments to determine the signaling pathways regulating transcriptional control of our gene of interest. We already have full length and truncated promoter...

02 June 2016 9,551 1 View

Does anyone know a simple method for removing differentiated cells from mouse ES cell cultures?

I have recently commenced a project involving in vitro differentiation of mouse ES cells into mesoderm and endothelium, and am the first person to establish ES cell culture in my laboratory....

07 October 2013 6,859 17 View

Does anyone have experience using cold-active proteases to digest tissues for single-cell RNA-Seq?

I'm hoping to get a cold digest protocol working in my laboratory to perform single-cell RNA-Seq on various mouse tissues, based on e.g. Potter et al. 2019 Methods Mol. Biol, digestion of mouse...

01 January 1970 3,973 2 View