Any recommendations on how to purify a midsize (~75 kDa) extracellular protein (hemocyanin) from a small amount of biological sample?

I'm trying to isolate and measure the concentration (absorption at 280) of hemocyanin from spider blood, but I am only able to reasonably get around 20-30 microliters of starting biological...

08 June 2016 1,431 5 View