Quynh Pham

4 Questions 2 Answers 0 Followers

Questions related from Quynh Pham

Why is my Urea PAGE 10% gel unable to separate the RNAs well?

I performed the RNase footprint with an 106-nt RNA. However, my hydrolysis ladder showed poor resolution from the >35-nt bands (attached figure). I used the 8M Urea gel with 10% AcrylamideBis...

09 February 2022 6,131 0 View

Why did my protein gel cannot be stained with silver staining properly?

I am doing silver staining since my protein concentration is low. However, the gel became dark and the protein bands appear as negative band (even the bands which are not present in coomassie...

21 June 2018 2,063 2 View

Using Nanodrop to estimate protein concentration shows high peak at 260 nm absorption?

I am using Nanodrop to measure protein concentration in lysate since it is fast and only requires small amount of sample. When I use water as blank, the result showed two high peak at 230 and 280...

08 November 2017 8,160 6 View

How many samples should we process to extract RNA at the same time?

I am using kits from Thermorscientific to extract total RNA. I extracted more than 20 samples and the results were various. Some of them had high concentration and the other ones were low. I do...

16 March 2015 9,486 4 View