Quyen Nguyen Thi

4 Questions 4 Answers 0 Followers

Questions related from Quyen Nguyen Thi

Could you suggest ways to improve the strength of a generated monoclonal antibody? Can we clone mAbs from infected host animals such as cows,.. ?

Dear everyone, We generated mAbs via hybridoma systems. The mAbs show high antigen binding affinity and specificty. However I still wonder what is the best way improve the strength of the mAb? To...

11 November 2019 5,922 3 View

Looking for a tumor cell expressing OVA protein on cell surface?

Dear all, I'm looking for a tumor cell line expressing OVA protein on the cell surface so that anti-OVA antibody in sera can bind to the cell through OVA protein. Does anyone has that cell line or...

06 June 2016 6,859 5 View

What is positive control for memory B cell ELISPOT assay?

Dear all, I'm going to quantify memory B cells using ELISPOT assay. I will use splenocytes from immunized C57BL/6 mice. Could you recommend any possitive control for this? Thank you very much!

02 February 2016 9,102 4 View

How can i convert O.D value from ELISA into titer?

Hello everyone! I did ELISA to quantify antibody production from sera (2-fold dilution from 1/100) without any standard. Now i want to convert O.D value into titers. Is there any suggestion for...

01 January 2016 7,818 3 View