Qun Ma

2 Questions 4 Answers 0 Followers

Questions related from Qun Ma

Can I use G418 and Zeocin together in E.coli and Pichia pastoris?

I am trying to use double selection marker, G418/kanR and BleoR in pPICZalpha plasmid. I am constructing the plasmid with both antibiotic resistance gene and clone it into E. coli Top10. However,...

05 July 2023 252 5 View

Why my colonies change from red to normal after I induce mcherry with IPTG?

Hello! I am using T7-mcherry plasmid in E. coli BL21(DE3). When I streak it on LBAmp plate, the colonies show beautiful purple-red color. When I streak it on LBAmp+0.1mM IPTG plate, the colonies...

27 April 2023 6,902 2 View