Qun Ma

4 Questions 6 Answers 0 Followers

Questions related from Qun Ma

Can I use Tn5 for transposon mutagenesis in an anaerobic Gram positive bacteria?

I plan to do transposon mutagenesis in an anaerobic Gram positive bacteria. The easiest way for transposon mutagenesis is the commercial Tn5 kit. I used that for E. coli before. But I didn't see...

12 September 2023 8,518 2 View

Can I use G418 and Zeocin together in E.coli and Pichia pastoris?

I am trying to use double selection marker, G418/kanR and BleoR in pPICZalpha plasmid. I am constructing the plasmid with both antibiotic resistance gene and clone it into E. coli Top10. However,...

05 July 2023 263 5 View

What's the distance between one terminator and the promoter for the next gene?

I am adding two genes into the same plasmid. I plan to make it as promoter1-gene1-terminator1--promoter2-gene2-terminator2. Does anyone know how many bp interspace should I leave between...

16 May 2023 2,111 3 View

Why my colonies change from red to normal after I induce mcherry with IPTG?

Hello! I am using T7-mcherry plasmid in E. coli BL21(DE3). When I streak it on LBAmp plate, the colonies show beautiful purple-red color. When I streak it on LBAmp+0.1mM IPTG plate, the colonies...

27 April 2023 6,908 2 View