Quentin Andres

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Questions related from Quentin Andres

For golden gate cloning is it better to PCR parts or to clone parts in entry vector and then digest ligate ?

I would like to assemble few constructs at the ORF level to make a fusion protein. Would it be preferable to use PCR to recover the parts from E.coli (recycling) and directly use them as...

10 October 2022 1,657 5 View

Using in vivo homologous recombination, would it be possible to obtain a high number of transformants by direct cloning in expression strain BL21?

In vivo cloning such as AQUA or IVA offer the possibility to rapidly clone fragments of DNA into E.coli based on short sequence homology (15-40bp), so the cloning can be easily done in high...

05 October 2022 3,850 2 View