Qu Zhang

2 Questions 8 Answers 0 Followers

Questions related from Qu Zhang

Best way to Dissociate Neuronal Spheroids into single cells?

Hi all, I am currently working on differentiating iPSC to neurons. A critical step of the process is dissociation of Neuronal Spheroids. I am using 0.05% Trypsin-EDTA, 37℃ for 20 mins, then stop...

14 August 2020 6,202 8 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

15 July 2020 3,985 7 View