Qingyun Zou

2 Questions 4 Answers 0 Followers

Questions related from Qingyun Zou

How to determine what is the proper level of overexpression?

Using siRNA, we have already found decrease of a protein can decrease growth factors-induced cell migration. For further study of its roles,We are planing overexpress this protein in primary...

20 June 2016 2,734 6 View

Why does the target protein level with siRNA-knockdown (on day 0) is reduced at day 2, rises back to control level at day 3 and drops again at day 4?

I performed a siRNA knockdown in vitro experiment. Target gene protein expression was decreased at 2 days after knockdown, however, it went back to normal level in compare with vehicle and ssRNA...

09 January 2015 9,874 3 View